Protein isolate is obtained by alkaline extraction and acid precipitation. The procedure is as follows: extraction of defatted meal with alkali liquor, centrifugal separation of indissoluble material in the meal (mainly polysaccharides), adjusting of leach liquor PH to the isoelectric point of protein with acid. When the protein achieves the isoelectric point, the protein coagulates and settles down. Finally, the protein precipitate after separation needs washing, neutralizing and drying.
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